Agriculture, Livestock & Forestry
  
HOME NEWS PICTURE GALLERY ABOUT US WHO IS WHO MORE...   Publications Program Profile Feed Back Login
 

GEARING NATION TOWARDS SELF SUFFICIENCY !!!

Research Center Bajo

Ministry of Agriculture, Royal Govt of Bhutan.

CEREALS

Rice LOW ALTITUDE
Rice HIGH ALTITUDE
Rice MIDDLE ALTITUDE
Rice RATOONING
Rice DOUBLE CROPPING
Rice SEED PRODUCTION
Wheat PRODUCTION

FRUITS & NUTS

Walnut PRODUCTION

VEGETABLES

Chilli PRODUCTION
Tomato PRODUCTION

LIVESTOCK

Pig PRODUCTION
Rice Fish CULTURE

FORESTRY

Forestry RESEARCH

SOIL/WATER MANAGEMENT

Maize TRASHLINE

PLANT PROTECTION

Pest & Diseases

Important Links

IRRI
Kuensel
Ministry of Agriculture
FAO
AGORA
CountrySTAT Bhutan
National Portal of Bhutan
CIMMYT
VERCON

 

Propagation & Management Techniques of Multi purpose trees species

1 Nursery Bed preparation for the Cuttings

Trying out vegetative propagation of potential agroforestry species is not so difficult provided the soil and nursery beds are prepared as per the plant species requirement. The vegetative propagation of plants is necessary for those species that are difficult to propagate through seeds. A fast and easy way to have successful propagation is by treating the cuttings with the root hormone, and this applies only to those plant species which are difficult to propagate through cutting. The temperature, humidity, soil moisture and soil aeration are some of the important factors to be considered because this has direct influence over success of propagation. These factors can be maintained through preparation of appropriate media and nursery micro sites. The choice of media depends on species some cuttings prefer only sand and some prefer only the soil while some may prefer the mixed ratio of sand, soil and sawdust. The cuttings usually require loose soil with high porosity.
The mixture of soil with the leaf mould sand and the sawdust is the perfect medium for cuttings. The sand helps in increasing the porosity, sawdust keep the soil moist and the leaf mould supplies the nutrition for the new sprouts.
Bed Preparation
1 Pit Bed
The pit bed is prepared by making the trench of 2-3 feet depth. Normally the width of the bed is kept 1 m for convenience in maintaining the cuttings. The length can vary as per the available space. Once the trench is complete filled it with stone gravels of 1-2 inches thickness to avoid water logging and improve aeration. Fill the prepared medium of sand, soil and the sawdust in the trench about 1 ½ to 2 feet thickness. Level the bed after filling and watered to make it complete wet. Plant the cuttings on the following day.
Cover the pit bed with the transparent plastic sheet for increasing humidity and moisture, maintaining temperature and to prevent from excessive evapo-transpiration either from the leaves or stems. The bed is ready for the plant cuttings. Construct shed using bamboo mats or any locally available materials to prevent it from direct heat, heavy rain, hailstone etc.
2. Raised Bed
There is not much difference in raised bed as compared to that of pit bed. The raised bed is constructed above the ground level at 2-3 feet height. The height of the bed is raised using the mud bricks, stonewalls or bamboo poles or any other locally available materials. The same medium used in both types of beds depending on the species requirement. The construction of shed and other requirements are same like pit beds.
The advantages of raised beds over pit beds are easiness and convenience in handling and maintaining the cuttings. It also prevents water logging. This is also good for those cuttings which require minimum humidity.
3. Polytube preparation

The mixture of sand, soil and leaf mould is made to the ratio of 1:2:2 and sieved to remove larger materials like stones, twigs etc. The

3
sieved soil is mixed thoroughly with the spade and shovel to have a good mixture. Sprinkle water to avoid dust. Check the medium by making a ball and rolling it over to find out the texture. If the ball breaks evenly while rolling, it is perfect, and if it doesn’t break the soil mixture is still clayey and needs addition of more sand and leaf mold. Prepared medium is filled in the polytubes of different sizes depending species. Two to three small holes are made at the base of the polytubes to drain excess water and also for aeration.

Fig: 1 Example of raised bed

4
2: Description, Propagation & Management of
Multipurpose tree Species (MPTS) species
I Scientific Name: Ficus bengalensis
Common name: Bar/ Wild banyan tree
Family: Moraceae
A Description
The commonly known as Bar (Lh) tree usually grows in the tropical foothills in our neighboring country India. In Bhutan it is found in the subtropical areas along Indo-Bhutan border from 200 to 1200 m above the sea level. The trees go up to 30 m height and have wide branching characteristics. The numerous aerial roots from the branches forming the additional prop differentiate the Banyan tree from the other Ficus species.
B Uses
The trees are planted along the roadside and near by the houses as a religious symbol in Hinduism. Due to the wide branching characteristic it is good for shed. The timber is used for making furniture and the foliage as fodder to cattle.
C Propagation Techniques
1 Seeds
2 Cuttings

1 Propagation through Seeds
Ficus bengalensis produces its figs annually in the month of September to October. The ripened figs are collected from the selected mother tree and the seeds are extracted through the maceration or through air-drying. The seeds are very sensitive to the insect attack due to the presence of sugar. Sow the seeds right after collection this increases the viability and to prevent from the insect attack.
Sow the seeds in the nursery tray or in the mother bed with prepared medium of sand and topsoil by broad casting. Once the seeds are broadcasted cover the seeds with thin layer of prepared soil medium. Water the seedbeds lightly with the watering can without making any splash.
3 Stem Cuttings

Stem cutting of this species is very successful even without the use of hormone unlike other tree species. The 1 – 2 cm diameter, and 6- 12 inches long stems from the mother tree is collected for the propagation. The collected planting materials are directly planted in the rooting medium. Approximately 1/4th portion of the cuttings are buried under the medium. Propagation is usually carried out on the on set of monsoon season just before the start of new sprouts. The root system will take place after 2-3 week from the time of planting. 100% survival is assured.

5
The rooted cuttings can be potted in the tubes for keeping it for long duration, or can be directly planted in the field.
II Botanical Name: Ficus roxburghii/ auriculata
Common Name: Fig Tree
Local Name: Nebaroo(Lh) Baku shing(Dz) Chongmashing(Sh)
Family: Moraceae
Description
This is a medium to big size tree commonly grown around the farmland as a priority fodder species. It grows from tropical to sub-temperate region from 200 to 2000 m above the sea level. It has big and rounded leaves and wider branching characteristics. The milk like sap oozes out from any wounded parts of the plant. The tree bears fig along the branches and the stem from June to September. The fig tree prefers to grow well along the gentle slopes under shaded and moist condition.
Uses
In Bhutan, farmers consider this as one of the important fodder tree species. The sweet red ripened figs are edible and children enjoy the fruit during the season. Big and rounded leaves are used for making leaf plates by some communities during rituals. It is also good for soil conservation due to its strong surface rooting system when planted along the edge of degraded or unproductive wasteland. The small size fuel woods can be obtained from the lopped branches.
Propagation techniques
Seeds
Cuttings

6
10 Steps to Extract clean Fig seeds.
1. The ripened figs are collected from the mother tree and splitted into two parts for extracting the seeds.
2. Inner layer of the fig bearing seeds is taken out with the help of small knife.

3. The fine sand particles are put in the bowl till ¾th of the size of container.
4. The seeds are mashed with hand and mixed thoroughly with sand by pouring little water.
5. Once it is mixed properly, the seeds along with sand are washed with water for several times.
6. This is done mainly to remove the sticky gum like substance from the seeds.
7. After thorough wash, water is filled till to the brim of the container and stirred with hand or sticks.
8. The minute seeds of the fig form the upper layer of the sand, the seeds can be poured in another empty container.

9. The process can be repeated till all the seeds are extracted.
10. The extracted seeds are wind dried for further storage and other uses.

Seeds
Due to the minute size of the seeds, it is very hard to germinate fig seeds like other tree seeds in normal condition. It is either eaten up by insects due to its sweet flavor or doesn’t germinate at all. Proper care and method is needed for propagation. The indigenous method of making a paste with cow dung along the walls has proven to be successful.
The extracted seeds are mixed with the fresh cow dung, making it like a paste. The pit bed is prepared under the nursery sheds or in the green house of 5 to 8 inches deep. The four walls of the bed are made slanting to 45 to 50 degrees. The walls are plastered with the mixture of cow dung and soil. Once the bed gets semi dried the prepared paste of seed is thinly plastered along the slanting walls and bottom of the bed.
The bed is kept moist till the seed starts germination. The ficus seeds usually take 2 to 3 weeks for complete germination. Around 80% to 90% germination percentage can be obtained through proper sowing methods and treatments. The 1-2 inch height seedlings are pricked out in the polytubes and kept under the sheds till it is fully established. After 2 to 3 months the potted seedlings can be taken out from the sheds for hardening.

7
The success rate of propagating Ficus roxburghii through stem cuttings is very less. The rooting to the stem is quite difficult due to the hollow inside the stem like that of the bamboo. Only 5 to 10 percent of the cuttings get survived. Therefore ideally it is not advisable to propagate Ficus roxburghii seedlings for mass propagation.
Field Plantation and Management
The 1 to 2 years old hardened Ficus roxburghii seedlings are ready for plantation in the field. The potted seedling has more potential to survive than the naked rooted seedlings. This could be because of the excess transpiration from the broad leaf of Ficus roxburghii. The naked rooted seedling gets wilted very fast before reaching the plantation site. The plantation is usually carried out during the onset of monsoon season. Watering is necessary for a week or two and even more, if the area is dry and under drought condition. The plantation needs clearing of weeds for at least two years to out-compete the grasses. The fencing or protection is needed to avoid the trampling and browsing of young saplings by cattle.
When the tree attains a height of 2 to 3 m, the terminal shoots can be cut off from the tip so as to allow more lateral branches to grow from the stems. Further the lateral branches can be trimmed off for further widening and increasing the crown size. This is done mainly for not letting the trees to grow taller which causes inconvenience while lopping for collecting fodder. It is also to increase the biomass production from the tree.
III. Scientific Name: Symplocos paniculata
Common Name: Asiatic sweetleaf
Local Name: Pangtse Shing
Family: Symplocaceae
A Description
The commonly called Pangtse Shing(Dz) around Wangdue and Punakha valley is identified as Symplocos paniculata in botanical term. It is a deciduous small tree or shrub up to height of 3 to 10 m. Symplocos paniculata grows in subtropical to temperate region at an altitude ranging from 1500 to 2850m above the sea level. Around Punakha valley the pangtse shing is usually found planted around the periphery of farmland for its edible oil. The plant bears flower from May to June and the seeds ripen from October to December. Natural regeneration is rare in the field. Farmers view it as one of the difficult species for propagation. Due to the oily nature of the seed it is hard to germinate in normal condition.
B Uses
Farmers of Punakha valley admire the plant for sweet vegetable oil extracted through its matured seed. Still today the farmers extract the pangtse seed oil through indigenous method. They say that the oil provides energy and taste is comparable to the other available vegetable oils in the market. It has also good medicinal value. Dye is also extracted from the plants. Environmentally the plant can be planted in the degraded or sloppy areas around the farmland to conserve the soil. The pruned branches and lops and tops can be used as fuel wood.

8
C Propagation Technique
1. Seed
2. Stem Cuttings
3. Root cuttings

1 Seed
The past research result reveals that it is really difficult to propagate pangtse shing through seeds. The different pre-treatment of the seed has been carried out to break the seed dormancy. In 2004 the matured seeds were collected from Hokona.
The berries were carefully harvested from the tree and brought to the nursery for careful study on germination. The seeds were extracted through the maceration from the fresh fleshy berries and were soaked in cold water to avoid the seeds from drying and losing viability. The seeds were given cold treatments by keeping the seeds in the deep freeze packed in the plastic bags. After two to three weeks of cold treatment the seeds were given pre-treatment of cold water soaking for at least 5-6 days.
Preparation of Medium
Unlike the seeds of other trees, panchi seed prefers the medium of continuous moist condition under the screen house. The medium could be prepared from the mixture of fine particles of sand, topsoil and sawdust with the ratio of 2:2:1.
Sowing method
The pretreated seeds can be sown in the mother bed either through dibbling, broadcasting or line sowing. The important thing to note while sowing panchi seeds is to maintain the depth of sowing which is almost 3-4 inches depth into the bed. The irrigation is frequently needed to maintain the moisture of the soil. The germination starts after 2-3 months of sowing. It is always good to sow the seeds right after collection and giving the recommended treatments for successful result. The seedlings can be potted in the plastic tubes once the true leaves appear. Keep the seedlings for at least one month under the shed to avoid direct light since the seedlings are tender and can be easily wilted.
Cuttings
2 Stem
The branches of thumb size and 10-12 inches long are collected as planting stock for root formation. The right season for the collection of cutting is during the dormant stage when the plant sheds its leaves and prepares for the new sprouting. In the field, the collected materials should be kept in the icebox or wrapped with the moist pieces of sacks or newspapers. This is done mainly to reduce the loss of moisture and to transport it or store for the longer duration. The cuttings can be now planted in the prepared medium to develop the root system. Root hormone can be applied to enhance the rooting system, and also to increase the success percent. Cutting usually prefers the warm and moist condition. In some cases the plant sprouts very well for a week or so and dies back without forming the root system, as the stored food inside the stem gets exhausted the plant dies. It usually happens when the cutting is done during the off season. It usually takes 4-5 weeks for the complete formation of root system.
3 Root
The propagation of panchi through root cutting is more successful than the stem. The process is same as that of the stem cutting, except the length of the cuttings is shorter than the stem cutting. The preferred lengths of 5-6 inches long are brought to the nursery and planted vertically with the base of the root upright. The ½ to 1-cm base length is exposed from the medium to give rise to new sprouts.

9
D Field plantation & Management
The one-year-old seedlings are ready to be transplanted in the field. The seedlings can be either planted out during dormant stage just before sprouting in the month of March, or during the onset of monsoon season. The panchi usually prefers moist condition, either along the bunds of terrace or in the unproductive land along the edge of fields. The seedling needs irrigation for 2-3 weeks after plantation in the drought condition. Weeding around the seedling is necessary for 2-3 seasons till it gets fully established. Young saplings need to be protected from cattle.
IV Scientific Name: Oroxylum indicum
Local Name: Tsampaka metog(Dz) Totola(h)
Family: Bignoniaceae
A Description
Tsampaka shing is a medium size tree that grows up to 5- 7 m tall. The tree belongs to the family Bignoniaceae. It is deciduous in nature and sheds the leaves in winter and sprouts when the monsoon
season starts. The tree is found in the tropical to sub-tropical region. It is characterized by thick fleshy and brown colored barks, with thick and pubescent leaves. The seeds are feathery and white in nature contained in the long pods. Due to the feathery nature of the seeds it is easily pollinated by wind in the natural forest.
B Distribution
In Bhutan tree is found in wild as well as domesticated in the farmland in the low to mid altitude level.
C Uses
Tsampaka metog is widely used by the Buddhist community for religious purposes. The Indians use this tree for the preparation of homeopathic medicines for the treatment of different kind of diseases.
D Propagation Techniques
Though we find less natural regeneration in the wild, still the plant can be easily propagated through seeds, roots and shoots cuttings in the nursery.
1 Seeds
The mature pods are collected in the month of April to May and the seeds are extracted by drying and splitting the pods. Seeds are sown in the month of May to June. It can be directly sown in the polytubes filled with the prepared soil or can also be sown in the mother beds

10
and later pricked out in the tubes when true leaves appear. The seed takes usually 2-3 weeks for germination. The simple pre-treatment can be applied by just soaking in the cold water for 6-12 hours to hasten the germination. The viable seeds of Oroxylum indicum give the 100% germination.
2 Cutting
The profuse growth of the new shoots from the spreading rooting system indicates the easy propagation of plants through cuttings. The roots and shoots are cut to the size of 10-15 cm and planted in the medium for rooting. The cuttings are usually carried out in the month of February – March when the tree is in dormant stage before the new leaf sprouts. The callus formation and rooting takes around 4- 5 weeks.
The plants can be potted once the rooting system fully develops.
E Field Plantation
Six months to one-year-old seedlings are planted out in the field during the on set of monsoon season. The pit size of 1 to 2 feet depth and 30 cm diameter is prepared for the plantation. The addition of fertilizer or farmyard manure would accelerate the growth of plants if planted in the degraded soil. The raised basin is prepared to avoid water logging and rotting of the tuberous roots.
F Management
Unlike other species Oroxylum indicum does not require many management regimes. During the initial stage watering for the first 2-3 weeks is required in the drought condition. Clearing of bushes around the plant could avoid the plants from shading and nutrition competition.
V Scientific Name: Benthamedia capitata
Local Name: Fetchi(Dz), Ramkattar(Lh)
Common Name: Duckwood
Family: Cornaceae
A Description
The Benthamedia species is a medium size tree that grows up to 5 to 10 m tall. There are two varieties identified locally according to the size and taste of the fruits. The leaves are opposite, entire and elliptic, and the fruits almost 3 cm in diameter with reddish in color.
It is usually found growing in the wild at mid altitudes along moist broad-leaved forest. The fruits ripen by the month of September to October.

11
B Distribution
The Benthamedia tree is usually found in the moist broad-leaved forest at an altitude range from 1830 to 2900 meters above the sea level. In Bhutan it is found in the districts of Chukha, Thimphu, Punakha, Trongsa, Bumthang, Mongar, Trashigang and Samdrupdzongkhar.
C Uses
The sweet edible fruit of fetchi is available in the Wangdue vegetable market during the spring season. The pulp of the fruit is sweet and edible. This could be one of the potential species for domestication as a fruit tree by the horticulture sector. During the flowering season of the plant it really beautifies the area with the pleasant smell from yellowish flower. Therefore the plant has an ornamental value too, and can be planted as an ornamental tree. The stem and branches can be used as fuel wood.
D Propagation Techniques
The stony nature of seeds of Benthamedia sp makes it difficult in germination in the natural condition. Moreover it takes a long duration to break the dormancy due to very hard seed coat. In the forest natural regeneration is observed in the place where birds and animals have eaten the fruits and excreted the undigested seeds. This is also counted as one of the pre-treatment of seeds swallowing by birds and animals. In the nursery condition the plants can be easily propagated through seeds as well as stem cuttings.
1 Propagation through seeds using different pre-treatments
The ripened fruit is collected from the tree and the seeds being extracted through maceration. The sweet pulp of the fruit is completely removed so as to protect the seeds from insect attack. The
seeds are air-dried and can be stored for a longer duration. The seeds usually take a year for germination without any pre-treatment. Different pre-treatment can be applied to hasten the germination. Here in the nursery we have tried out with three different pre-treatments namely Scarifying, Hot water and Cold water treatment. The scarifying is removing the apex of the seeds with the help of Scrature or nail cutter without damaging the embryo. The scarified seeds are sown in the mother bed through dibbling or line sowing methods. The germination is observed after three weeks of sowing. The hot water pre-treatment is by soaking the seeds in the boiling water for minute and soaking it again in the cold water for 12-24 hours. The hot water pre-treated seed takes a month for germination. The cold water pre-treatment is simply soaking the seeds in the cold water for 24 to 48 hours and changing the fresh water at intervals. Other method to hasten the germination of Benthamedia seeds is by sowing the seeds in the mother bed or germination box with the medium of only fine particles of sand.
Sl.No Pre-treatments Germination period Germination
percent
1 Scarification 2-3 weeks 95%
2 Hot water 4-5weeks 70-75%
3 Cold water 6-8 weeks 60-70 %
4 Sand bed 4-5 weeks 80%

2 Cutttings
The stem cutting of this species is very successful. The consideration to be taken is the right season for planting of cuttings and in the prepared medium of sand, soil and sawdust inside the green house or in the Polly tunnel / misting chamber. Usually cuttings are preferable before the sprouting of the new shoots. The rooting system develops after a month or two if planted without treating of root hormones, but

12
with the application of root hormones the rooting starts after 2-3 weeks. The rooted plants can be then planted in the polytubes for keeping it in the nurseries till the planting season.
E Nursery management and planting out
One year old seedlings are planted out in the field after hardening the seedlings for few months. The size of the pit is prepared same as that of other tree species. It prefers moist soil and is light demanding. The care for few months is necessary to fully establish the growth of the trees. The shape and the size of the crown can be maintained through proper pruning and thinning of the branches.
VI Scientific Name: Thysanolaena latifolia
Common Name: Tiger grass
Local Name: Tsakusha (Dz), Beyshaw(Sh)
and Amlisho (Lh).
Family: Gramineae
Sub Family: Thysanolaenae
A Descrription
It is a bushy rhyzomatous perennial grass and it’s widely distributed in the country (from 200m asl to 1800m asl). It grows as high as 2-3 m tall. The leaves are linear about 5 cm wide and 30-35 cm long which are alternately arranged. After a year of plantation it starts producing the panicles in which the inflorescence is borne along an elongated axis. The plant is usually found growing along the roadsides and in the difficult terrain areas.
B Uses
It has many uses: a) the grass for fodder (fig 1); b) infloral parts as broom (fig 2) and c) its roots for the medicines. Farmers particularly in the southern part of the country grow it along the ridges of their field and in wastelands around the homestead. Besides these use values, the plant serves environmental functions such as soil stabilization in the degraded areas, erosion control in the hilly areas as it grows in clumps and multiplies rapidly through rhizomes. The plant as such has a lot of potential for Agroforestry.
C Propagation Technique
Propagating through rhizomes is very simple and effective with planting in the rainy season. The rhizomes should be collected by manually digging from any good source but remembering not to collect rhizomes from those in the steep areas and roadsides to prevent soil erosion.

13
Shoots of the clumps should be cut off at about 6 to 7 inches from the dug rhizome. The collected clumps are then directly planted or else taken to the nursery for generating more planting materials (fig 3).
D Multiplication in the Nursery
These clumps of rhizomes can be separated into the single rhizome with the help of a knife or a pair of secatuers. The single rhizome should have the rooting system and possess tender buds.
A raised bed (1x2 feet) should be prepared although the length of the bed can depend on size of the available plot onto which graded and selected single rhizome are planted with a distance of 1m spacing between them. Leaf mould and other organic fertilizer should be added for rapid growth of new shoots. Planting rhizomes on the raised bed makes it easier to dig out for further planting in the field. It also eases in watering and helps out-compete weeds. Single rhizomes develop into a clump of 8 to 10 new shoots and each shoot becomes a rhizome itself in a year. The plant should start flowering within a year and be used for various purposes.
E Method of planting in the field
The tiger grass rootstocks/rhizomes can be directly planted in the field as required. The planting hole of 50 – 60 cm diameters is prepared in the planting site. The site should be cleared of weeds through strip clearing or spot-ring/patch clearing method. The culm of rhizome is collected and each culm can be divided into 4 planting rootstocks for plantation. The plantation is carried out usually on the on set of monsoon season to acquire more survival rate. The space for planting tiger grass depends upon the objective of plantation. If the target is for production of panicles wider space is required and if for conservation practices planting in close space would be more effective. The

Clump of rhizome

Single slip separated from the clump

14
fertilization could be done in the areas where soil nutrients is deficient to enhance the growth of the plants
F Some management aspects
The plant does not demand sophisticated management regimes. In the nursery it is easy to propagate and requires watering only during the dry days.
In plantations occasional weeding should be done especially during the initial establishment stage and if there is lot of vegetation growing around. Once the plant gets fully established the size of the clumps becomes large and can hold the soil strongly preventing landslides and soil erosion.
Once fully grown (i.e. within a year) the foliage can be cut and carried home as fodder and the infloral parts used either as a soft broom or for other purposes. The plant is then cut off from the height of 5 to 6 inches from the ground, which can serve as fodder during the winter months. The cutoff clumps of Thysonalena can be left as it is for new shoots or else burnt which encourages profuse growth of new shoots when the wet season arrives. Because of its profuse regenerative capacity (fig 4) and robust growth it is important not to plant the tiger grass inside the agricultural field to avoid dominating agricultural crops and becoming a difficult weed. The plant is very effective in soil conservation if planted along contours along the steep slopes where the agricultural crops cannot be grown.
VII Botanical Name: Cupressuss corneyana
Common Name: Cypress tree
Local Name: Tshenden Shing(Dz) Dhupi (Lh)
Family: Cupressaceae
Description
Cupressuss corneyana Carriere; C. funebris F.B.I. non Endlicher (Tsenden –Dz-; Weeping Cypress -Eng) is one of the most important native conifer species in the country growing between 1000-3000 m asl. The species has historical religious and cultural significance with old Tsenden trees seen grown in the periphery of monasteries and Dzongs. It is also the National Tree of Bhutan.
Uses
Owing to its religious connection and the high quality timber from the species it is used as for construction and renovation of Dzongs and Monastries. The leaves of the Tsenden gives sweet aroma when burnt so it is used as incense with whole branches and leaves burnt.

15
Figure 1: Prime timber from Tsenden
It is also planted as ornamental plant because of its bushy conical shape. Its deep rooting system helps bind soils together. It is relatively fast growing and a preferred species for planting by farmers so has huge potential as an agroforestry species.
Figure 2: Tsenden as a plantation species
10 Steps for propagating Tsenden
1. Seeds should be collected from superior mother trees in Sept-Nov. Usual collection areas in Wangdue are Chuzelumpa and Dangchu. Alternatively you can request small amount of seeds from the DFO, Wangdue.

2. Spread collected seeds thinly on a tray (or bamboo mat) to allow air to move freely over it to dry and stored in an airtight container in a cool place.

3. Before sowing, soak seeds in cold water for about 12-24 hours to break dormancy and achieve better germination rates.
4. Sow seeds in beds inside screen house if available or shaded mother beds (1 m wide with alleys 0.5 m in between) at a depth of about 3 mm in rows with 3 cm distance apart. The distance is to ease weeding and pricking out.

Figure 3: Tseden seedlings in shaded mother bed
5. Sown seeds should be watered daily until the seedling grows to a height of 2 cm (in areas as dry as Bajo), in wetter areas the mother beds can be prepared to allow sufficient drainage to avoid damping-off to the seedlings. Use a watering can with a long delivery trunk to avoid disturbing the sown seeds while watering.

6. Before pricking out prepare growing mixture by using equal proportions of soil, leaf mould and sand. Poly tubes should be filled up at least 3 days before pricking out. Leave a gap of about 1 cm to1.5 cm from the soil surface to the top of the tube for watering and refilling.


16
Figure 4: Use a watering can and water regularly
7. Use of polythene tubes is advised as bare roots have lower survival rates. Punch holes in the bottom of the poly tubes before filling with the growing mixture to provide sufficient percolation of water (prevent water logging) and aeration.

8. Prick out seedlings at a height of about 2 cm to minimize pricking out shock. Pricking should be done carefully. Use a small garden shovel for the purpose.

9. In case there is an occurrence of fungal diseases then arrange tubes with seedlings and without in alternate rows.

10. Harden seedlings by removing all shade and reducing the frequency of watering.

Figure 5: Nursery shed give extra protection for successful germination
Management and Field Plantation
Lifting seedlings from the nursery for planting forms a critical part for successful survival. Avoid damaging the plant particularly the roots when lifting from the nursery. Grade seedlings according to their height and robustness and plant into small pits dug in the planting area at 2.5 m x 2.5 m. watering is crucial for about 2 weeks especially in dry areas during establishment phase. Tsenden growth is highly stunted in areas with a high degree of exposure. In such areas fast growing trees should be planted as nurse trees.
Figure 6: First harvest from a Tsenden stand
VIII Botanical Name: Bambusa & Dendrocalamus spp.
Family: Gramineae
Local Name: Bans(Lh) Pashi(Dz)

17
Introduction
Bamboos commonly grown as woody plants, belong to the family Gramineae, and form the tribe Bambuseae of the subfamily Bambusoideae. They often have tree like habit and can be characterized as having woody, usually hollow clumps, complex rhizome and branch systems, petiolate leaf blades and prominent sheathing organs. Bamboo species are distributed throughout the country, though they are most common in the higher rainfall areas. They can be found from Tropical Southern borders to the tree line where they form extensive areas of pasture for higher altitude cattle like yak and sheep. Bamboo remains one of the most important multipurpose plants in the Bhutanese society. Traditional ways of bamboo propagation are not well documented and less frequently understood. Hence it was felt important to study and document propagation methods for both local and exotic species. The propagation activities were initiated from 1996 and it are still on going.
Uses
Bamboo has multiple uses in the Bhutanese community (e.g. roof mats, fencing pole and post, canning of shoots of edible species, building construction, basket, etc.).Bamboos also play an important role in soil conservation by virtue of their dense surface root, which helps protect from sheet and gully erosion. It can be planted in slope and landslide areas for stabilization. It can also be planted along the agricultural field boundary and home garden as a windbreak to protect crops.
Methods of propagation
Usually there are three propagation techniques being practiced. Farmers collect the rhizomes from the source and directly plant in their fields. Some also collect the shoots of mature clumps keeping the internodes of two to three numbers and burying under the soil till it gives out the new shoots and roots. The other commonly practiced and effective method is raising through the seeds, which is usually practiced in the nursery for mass multiplication. Here we discuss mainly on techniques through the seeds and mass production of planting materials.
1 Through seed
2 Rhizome
3 Culm cutting
4 Branch cutting
5 Tissue culture

Nursery Method
• Raised bed
• Direct seeding in the plastic tube

Propagation from seed
This method is effective and useful especially if we are to produce a large numbers of field plantable bamboo seedlings. The technique and the methods involved are as follows.
Seed treatment
Soak the seed in cool water for 10-12 hours and sow it on well prepared seed bed or in the plastic tubes.
Sowing
Two types of sowing are commonly done
• In plastic tube and in
• Seed bed

Put 3-4 seeds to each plastic tube and cover the seeds by using soil, sand, and top soil up to 5 mm.
Sowing in the seedbed
In the prepared seedbed sow the seeds thinly and cover with soil mixture. Sowing too thick may hamper rhizome formation.
Substrate
Mixture of soil, sand and top soil in the ratio of 1:1:1

18
Watering
Maintaining good moisture and timely watering is critical even before and after germination.
Protection
Application of pesticides helps to keep away pest against seed damage in the nursery.
After germination, rhizome formation will take place in 3 months time. From each of these seed based seedlings, young shoots as many as 4-5 are detached and transplanted. Each of thus produced seedlings develops shoots which can be separated for transplanting. From 100 seedlings with the detachment of 3 shoots from each could easily raise 300 seedlings within a year. This process could continue without having to procure seeds for the second time and the mortality rate of such seedlings is as low as 3-4%. This method has proved successfully with Dendrocalamus strictus, Dendrocalamus calastachyous, Bambusa bamboo and Bambusa arundinacea.
Plus point
1 Multiplication through seeds is a simple method for mass
propagation of Dendrocalamus strictus, Bambusa bamboo and Dendrocalamus for raising large-scale planting

2 This method ensures that each propagule has shoot, root and rhizomes even at the time of tiller separation, enabling rapid establishment and very high survival of propagated materials.

3 The dependence on bamboo seed production in nature is totally eliminated from second year onwards for mass production of field plantable bamboo propagules. The method developed is simple, easy and involves use of locally available materials.

4 Seeds are sown between March and July with the pricking done when seedlings have attained a height of about 5-10 cm (i.e. about April-August).

5 By the last week of May the next year, about five to six tillers on an average are produced in each plastic tube.

6 Cutting rhizomes for production of more propagules separates these tillers. After separation, each propagule is planted in the plastic tube for further growth and development.

7 By July the following year, a number of propagules for each species are retained in the nursery for production of more propagules next year. The process can be repeated depending on the amount of seedling required.


19
IX Scientific Name: Phylanthus emblica
Common Name: Gooseberry
Local Name: Omla(Dz), Amala(Lh), Chhorgeng(Sha)
Family: Euphorbiaceae
A Description
This is a shrub or medium size tree from 1 to 10 m tall medically termed as “Churoo”. It is a subtropical plant that usually grows in the warm broad-leaved and chirpine forests. The plant is a light demanding species and grows well in the dry and sunny slopes. The growing elevation ranges from 300 to 1800 m above the sea level. It starts bearing fruits from September and remains till March.
B Uses
The Omla tree has a very high demand in the preparation of indigenous medicine. The sour taste berries of Omla are believed to have high content of Vitamin C. The berries are used to extract Amala hair oil in India and lots of other pharmaceutical preparations. In Bhutan, the farmers sell ripened berries in the market as horticultural fruits for cash income and also used as tannin. In some areas local pickle is prepared through fermentation in the salt and mustard oil. The twigs and branches can be used as a fuel wood. The plant can also be used to rehabilitate the dry and sloppy unproductive areas.
C Propagation Techniques
a) Seeds
b) Stem Cuttings

a) Seeds

The ripened berries of Phylsanthus emblica are collected from the tree and let them dry in the sun to break the pods. The fleshy parts of the fruits can either be pealed off for immediate extraction of the seeds or can be sun dried which usually takes a bit longer. The seed numbers range from 5 to 6 per fruit. The viability of the seed is very high and can be stored for one to two years if properly packed. The seeds can be sown from the month of April and under the screen house any time of the year. The germination time differs with the application of various pre-treatments. The pre-treatment of the seed is necessary to hasten the germination due to the nature of the hard seed coat. Seeds can be either treated with soaking in hot water, cold water, and acids or through sand stratification. It takes around a week or two to germinate the seeds pre-treated with hot water, acids and sand stratification, and for a month or two with cold water, and for 6 months to year without any pre-treatments. The seedlings are pricked out in the polytubes from 1-2 inches height and kept under the sheds till they get established. The saplings are exposed for hardening few months before transplantation.
b) Stem Cuttings

The thumb size girth and 6 to 8 inches long stems are collected for carrying out propagation. The stem cutting is carried out during the onset of monsoon season or at the dormant stage of the plant before sprouting. The one-year-old shoots are better for cuttings, and have a higher success rate. The cuttings are planted in the prepared medium for rooting. After 2-3 weeks, the rooting system develops and can be pricked out in the tubes. The use of hormones could shorten the time for the formation of roots due to faster callus formation.
Field Plantation and Management
The hardened seedlings of 1 to 1 ½ years are planted in the field. The shape and size of the planting pit is prepared as that of the other tree species. The Omla tree prefers open sloppy areas due to its light demanding characteristics. Irrigation is necessary for first few weeks to supplement the loss of water through excess transpiration and also to establish the plantation. The planted seedlings have to be protected from cattle to avoid trampling and browsing damage. Clearing of grasses and weeding is crucial for first few years to allow the free growth of plants and avoid suppression and to reduce growth competition with weeds.

20


Copyrights © Bajo Research Center,Ministry of Agriculture Bhutan

Hit Counter